Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine . The authors suspect that U87MG is the most cell that is popular created, with approximately 200 studies posted in 2015 alone . It is reported to produce a malignant tumour consistent with glioblastoma in nude mice. Bioz Stars score: 86/100, based on 1 PubMed citations. 34 BW134246. Med. Misidentified cell lines are still being reported, occasionally, with the cognizance of the authors . that the U87MG cell line from ATCC had a different . Two groups constituted the controls. 47,48 There were seven genes differentially expressed more than twofold in U87MG cell line after exposed to As 2 O 3, of which five genes were upregulated and two genes were downregulated. . "The Uppsala cell line was genetically identical with the original tumor whereas the U87MG cell line from ATCC had a different, unknown origin," senior author Bengt Westermark, an immunology, genetics, and pathology researcher at Uppsala University, said in a statement. v TABLE OF CONTENTS ACKNOWLEDGEMENTS iii ABSTRACT v TABLE OF CONTENTS vi LIST OF TABLES ix These data are consistent with our studies of JCV replication, gene transcription and virion production in PHFG cells. The authors, whose laboratory developed the cell line almost 50 years ago, compared the genetics of this line (obtained from ATCC) with those of the original tumor. to study the mechanism of Pax6 (paired box protein)-associated increase in expression of Dkk3 (Dickkopf 3) to study the role of JARID1B (jumonji AT-rich interactive domain 1B) in the pathogenesis of glioma. Therefore, it is advisable to authenticate a cell line prior to any experimentation. indicate that the cell line presented in the work is of (or likely of) glioblastoma . Science Translational Medicine 31 Aug 2016: Vol. Trans. ZERO BIAS - scores, article reviews, protocol conditions and more Our dats suggests that U87MG glioblastoma cell line will expedite the study on polyomavirus JC. Allen M, Bjerke M, Edlund H, Nelander S and Westermark B: Origin of the U87MG glioma cell line: Good news and bad news. Lipofectamine 3000 reagent outperforms Invitrogen Lipofectamine 2000 and FuGENE HD reagents. The original U87MG cell line was established in our laboratory almost 50 years ago ( 1 ). reveals that the U87 cell line used in this study is distinct from the original U87MG cell line [].Additional analyses by Allen et al. U87 cell line used in the present study matched the U87MG cell from ATCC. The tumor cell lines of mouse origin can also be injected into normal immunocompetent mice, as long as they are of the same mouse strain 34 BW134246. Vol. One consisted of animals implanted with cells growth medium, whilst the second included naive normal rats. Clark MJ, et al. Place, publisher, year, edition, pages 2016. Targeting of the human epithelial receptor type 2 (HER2)-neu receptor by specific antibodies is a recent well-established therapy for breast tumours. All evidence considered, this study exposes further phenotypic Together with a few additional glioma cell lines, including the widely used U251MG and U373MG, the U87MG line was deposited in a cell repository managed by Fogh et al. Cell lines are therefore indispensable in medical research and a large number of cell lines exist that originate from many different tumor types. Amid the ongoing controversy over the origin of the ATCC U87MG cell line, ATCC reported commercial U87MG is of central nerve system origin, with an unknown glioma patient derived origin 32. U87MG cells from a glioblastoma line of human origin caused a single irreversible wave of aggregation simultaneously with the onset of platelet secretion, and this was inhibited by heparin and . 8(354): 1-4, 2016. All experiments were performed with cells in the logarithmic growth phase. I. C. Chondroitin sulphate-modified neuropilin 1 is expressed in human tumour cells and modulates 3D invasion in the U87MG human glioblastoma cell line through a p130Cas-mediated pathway. Trans. The expressions of the GSC markers CD133 and nestin were detected using immunocytochemistry to identify GSCs. They have an infi- MATERIALS AND METHODS Materials Cell lines U87MG, U373MG, U1I8MG, A172, H4, SW1088, SW1783, HS683, CCF-STTGI,IMR-32,SKNSH, SKNMC, T98G, U138, and 7TD1 were obtained from the American Type Culture Collection, U.S.A. Dulbecco . 'The comparison showed that the Uppsala cell line was genetically identical with the original tumor whereas the U87MG cell line from ATCC had a different, unknown origin. Questioning the authenticity of the cell line, Westermark et al. All cell lines were used between the 10 th and 30 th passage. The expressions of the GSC markers CD133 and nestin were detected using immunocytochemistry to identify GSCs. It is also deadly, with a survival rate of just 36.5% in the first year following diagnosis. 32 Production of Progeny Virus The glioblastoma cell line, U87MG was transfected with JCV molecular clone and lysed on day 14 p.t and na'ive primary human fetal glial cells and U87MG glioblastoma cells were infected with the above lysate and DNA was extracted on days 0, 3, 5, 8 and 14 and viral replication was confirmed using the method . A special feature of this paper is that the team responsible for the work is performing it in the same laboratory that developed the line nearly 50 years ago, in 1968. We find that the DNA profile of the widely used glioma cell line U87MG is different from that of the original cells and that it is likely to be a bona fide human glioblastoma cell line of unknown origin. The human ovarian carcinoma cell line SKOV-3, the human breast carcinoma cell line MDA-MB-231, the human glioblastoma cell line U87MG, and the human prostate carcinoma cell line LNCaP were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and cultured as previously detailed []. The cell line distributed by most cell collections (including ATCC, CLS, ECACC, etc.) . Formally known as U-87 MG (abbreviation for Uppsala 87 Malignant Glioma), the U87 cell line has an epithelial morphology and was obtained from a 44-year-old female patient in 1966 at Uppsala University. Med. Two different mechanisms of aggregation of heparinized human platelet-rich plasma have been identified with two tumor cell lines: In neither case are these mechanisms dependent on platelet-derived ADP. COX-2 expression and activity in GBM cell lines. As an internal control in these studies of U87MG microvesicles we have used the HL-60 promyelocytic leukemia line, which is known to contain procoagulant tissue factor activity. This cell line is not the original glioblastoma cell line established in 1968 at the University of Uppsala. The TMZ-resistant subclones . 'The comparison showed that the Uppsala cell line was genetically identical with the original tumour whereas the U87MG cell line from ATCC had a different, unknown origin. Place, publisher, year, edition, pages 2016. Cancer Cell Lines and Substrates Caution: For Research Use. To cite this cell line use: U-87MG ATCC (RRID:CVCL_0022) Comments. Using genetic analyses, the researchers showed that a commonly used cell line which was established in Uppsala almost fifty years ago does not origina.. U87MG: Not what it used to be A cell line commonly used for research on gliomas is found to be different from the original tumor from which it was derived. Resazurin reduction assay Resazurin reduction assay [56] was performed to assess the cytotoxicity of the studied samples toward various sen-sitive and resistant cancer cell lines. ATCC cell culture human glioblastoma u87mg cell line Cell Culture Human Glioblastoma U87mg Cell Line, supplied by ATCC, used in various techniques. The relationship between cell surface sialylation and platelet-activating activity was studied in two tumor cell lines of human origin, the SKNMC neuroblastoma line and the U87MG glioblastoma line. At 72 h after infection, 2 g/ml puromycin was used to screen cells overexpressing ITGA5, named U87MG-ITGA5. Origin of the U87MG glioma cell line: Good news and bad news Marie Allen,1 Mia Bjerke,1,2 Hanna Edlund,1,3 Sven Nelander,1 Bengt Westermark1* Human tumor-derived cell lines are indispensable tools for basic and translational oncology. U87MG cells from a glioblastoma line of human origin caused a single irreversible wave of aggregation simultaneously with the onset of platelet secretion, and this was inhibited by heparin and hirudin but not by apyrase or phospholipase D. Trans. The effect of TMZ on the viability of glioblastoma cell line (U87MG) After 24 h of incubation, TMZ at concentrations 10 M-50 M did not significantly alter the viability of the U87MG cell line (Figure 1).The marked reduction of viable cell number (79% of control) at that time point was observed only when TMZ was used in concentration of 100 M. We find that the DNA profile of the widely used glioma cell line U87MG is different from that of the original cells and that it is likely to be a bona fide human glioblastoma cell line of unknown. Sci. Species: Cell viability of K562, U87MG and HT29 were evaluated by means of the Cell Counting Kit-8 (CCK-8) (Dojindo Laboratories, Kumamoto . As described in PubMed= 27582061 it is most probably also a glioblastoma cell line but whose origin is unknow. Likewise, the GBM 6, SF-767, U87MG, HK301 and BAH1 lines shared the presence of the 16519 bp variant at high levels (range 51.2 to 58.2%), whilst the GBM 6 and U87MG cell lines shared the presence of the 3168 bp variant (16 s rRNA) at a frequency of 3.7%. indicate that the cell line presented in the work is of (or likely of) glioblastoma . Also Known As: 'The comparison showed that the Uppsala cell line was genetically identical with the original tumor whereas the U87MG cell line from ATCC had a different, unknown origin. A recent study published in Science Translational Medicine has reported that the glioblastoma cell line, U87MG, has a different genetic profile to the original tumour it was isolated from almost 50 years ago. from the U87MG glioblastoma cell line of unknown origin. Glioblastoma multiforme (GBM) remains the most devastating primary tumour in neuro-oncology. However, although both U87MG and U251MG are glioblastoma cell lines, they are 88 actually distinct cell types with completely different sources of origin, such that their 89 genetic differences extend far beyond just p53 (Camphausen et al., 2005). Glioblastoma Cell Line Biobank: A New Resource. U87MG is a commonly studied grade IV glioma cell line that has been analyzed in at least 1,700 publications over four decades. They have an infinite life span and are easy to handle and scalable, and results can be obtained with high reproducibility. lL-6and IL-6Rare expressed in fetal brain but not in adult. In addition, the differentiation potency of these GSCs was observed by detecting the expression of glial fibrillary acidic protein, -tubulin III and galactosylceramidase using immu- It has been reported that the U87MG cell line from ATCC is of CNS origin and is likely to be derived from another patient with glioma, although Glioblastoma cell line U87MG is a well-established cell line that widely used in glioma research, but few focused on the change of its biological characteristics during serial passage. "We find that the DNA profile of the widely used glioma cell line U87MG is different from that of the original cells and that it is likely to be a bona fide human glioblastoma cell line of unknown. Researchers studying the brain tumor type glioma often use a cell line called U87MG that was established at Uppsala University almost fifty years ago. Passaging U87MG revealed the presence of chromosomal anomalies reflective of structural genomic alterations in this glioblastoma cell line. suppressed anti-apoptotic signals and activated cysteine proteases . 8(354): 1-4, 2016. Anti-oncogenic activities exhibited by paracrine factors of MSCs can be mediated by modulation of KITLG and DKK1 genes in glioma SCs in vitro A new paper published in Science Translational Medicine and led by Bengt Westermark has attempted to tackle these questions with regard to a very common and widely used cell line in cancer research, specifically one called U87MG. We find that the DNA profile of the widely used glioma cell line U87MG is different from that of the original cells and that it is likely to be a bona fide human glioblastoma cell line of unknown origin. Nelander S, Westermark B. Huh-7 Origin The HuH-7 cell line was established in 1982 from a well differentiated hepatocyte derived cellular carcinoma cell line that was originally taken from a liver tumor in a 57-year-old Japanese male. The relationship between cell surface sialylation and platelet-activating activity was studied in two tumor cell lines of human origin, the SKNMC neuroblastoma line and the U87MG glioblastoma line. Read moreGeneral Information Three of them were intracranially implanted with human GBM cells of different origin (U87mg and T98g cell lines and patient-derived glioma cells) suspended in the culture medium. Trans. This product is intended for animal research only and not for use in humans. Caution: This is the original U-87MG cell line established in 1968 at the University of Uppsala. For the TMZ-resistant GBM cell line, U87MG cells were exposed to the IC 50 of TMZ (HY-17364; MedChemExpress, Monmouth Junction, NJ, USA) and then treated continuously with the IC 50 of TMZ for 3 months. This analysis revealed that the origin of the cell line is . Cell Line Description Derived from a malignant glioma from a female patient by explant technique. Origin: Derived from a malignant glioma from a female patient by explant technique . This averages out the impact . Origin of the U87MG glioma cell line: Good news and bad news. blastoma cell lines U87MG, U373MG, and UI 18MG is documented. vironment. utilized forensic and DNA that is mitochondrial to locate U87MG's origin. Origin: Derived from a malignant glioma from a female patient by explant technique . In addition, the differentiation potency of these GSCs was observed by detecting the expression of glial fibrillary acidic protein, -tubulin III and galactosylceramidase using immu- is a different cell line whose origin is not yet clear (PubMed= 27582061 ). I. C. Chondroitin sulphate-modified neuropilin 1 is expressed in human tumour cells and modulates 3D invasion in the U87MG human glioblastoma cell line through a p130Cas-mediated pathway. U87MG decoded: The genomic sequence of a cytogenetically aberrant human cancer cell line. Origin of the U87MG glioma cell line: Good news and bad news. . We have examined these questions using genetic profiling and transcriptome analysis in human glioma cell lines. Glioblastoma is the most common primary malignant brain tumor in adults. The tumor cell lines of mouse origin can also be injected into normal immunocompetent mice, as long as they are of the same mouse strain Clark MJ, et al. Origin of the U87MG glioma cell line: Good news and bad news. This product is intended for animal research only and not for use in humans. . 8, no 354, article id 354re3 National Category Cell and Molecular Biology Cell viability was assessed after 24, 48 and 72 hr of continuous exposure with the different compounds described above. Problematic cell line: Misidentified. PLoS Genetics 6 (1) : e1000832, 2010. . Sci. Cell lines and in vitro exposure to MF. (2016) Origin of the U87MG glioma cell line: Good news and bad news. The use of alkylating agents such as temozolomide in association with radiotherapy (RT) is the therapeutic standard of glioblastoma (GBM). Thus, transfected clones are monoclonal in terms of their single cell origin from the U87MG cell line, but polyclonal as related to the transfection with GFP or RFP. See U-87MG ATCC ( CVCL_0022) for the cell line used by most research groups. Positive control cell line SW71 showed the highest binding percentage of ~96 % while negative control PBMC cells showed a non-specific binding. The cell line was thought to be deposited at the Memorial Sloan Kettering Cancer Center in 1973, after which the ATCC obtained it in 1982. . Human epithelial . Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours posttransfection. from the U87MG glioblastoma cell line of unknown origin. . Cell culture and in vitroexposure to mifepristone. Med. Cancer Cell Lines and Substrates Caution: For Research Use. Sci. U87MG, HCT116 (p53+/+), HepG2 and AML12 cell lines. 8(354): 1-4, 2016. The line was established by Nakabayshi, H. and Sato, J. Huh-7 is highly susceptible to the hepatitis C virus (HCV), and it . Even cell lines maintained at ATCC might not be authentic, as in the case of E006AA or widely used glioma cell line U87MG . DNA profile of the widely used glioma cell line U87MG is different from that of the original cells and that it is likely to be a bona fide human glioblastoma cell line of unknown origin 1). Karyotyping confirmed the female origin of U87MG sourced from Europe. reveals that the U87 cell line used in this study is distinct from the original U87MG cell line [].Additional analyses by Allen et al. PLoS Genetics 6 (1) : e1000832, 2010. . Origin of the U87MG glioma cell line: Good news and bad news. This Research Article [] reports the sequencing of "U87MG" cells, which were acquired from ATCC.It has come to the authors' attention that recent work from Allen et al. The cells were passaged twice weekly. Sci. Thus, even 90 though many studies on p53 are performed on these two cell lines due to the known For U87MG cells, the percentage binding with mAb was ~66 % (corresponding figure is given in supplement material). This Research Article [] reports the sequencing of "U87MG" cells, which were acquired from ATCC.It has come to the authors' attention that recent work from Allen et al. Article Snippet: Cell lines and primary cultured glioblastoma cells U87MG (TP53 wild-type) and T98G (TP53 mutant, M237I) glioma cell lines were obtained from the American Type Culture Collection and cultured with DMEM with 10% fetal calf serum (FCS) supplemented with L-glutamine (2 mM) and penicillin-streptomycin (100 U/ml-100 g/ml). Bioware Brite U87MG-Red-FLuc EMEM+10% Hyclone FBS. Human GBM cell line , U87 MG, was cultured on a series of HMC-agarose based culture system. 8, no 354, article id 354re3 National Category Cell and Molecular Biology Identifiers All evidence considered, this study exposes further phenotypic nuances of U87MG which may belie researchers seeking data contributing towards the elusive cure for GBM. In order to comprehensively characterize the genome of this cell line and to serve as a model of broad cancer genome sequencing, we have generated greater than 30 genomic sequence coverage using a novel 50-base mate . U-251 MG cell line human has been used: for the extraction of cancer stem cells from U-251 human glioblastoma cell line. Med. Bioware Brite U87MG-Red-FLuc EMEM+10% Hyclone FBS. Studies to improve survival are hindered by imperfect cell models of glioblastoma. 8(354): 1-4, 2016 Derived from a malignant glioma from a female patient by explant technique. 13, 14 In the present work, it was demonstrated that both the plasma membrane and cell-free microvesicular fractions from HL-60 cells showed procoagulant activity similar to that seen with U87MG but with less than one . Application U-87 MG cell line has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. Science Translational Medicine in U87MG glioblastoma cells. Cell Line Description: Important information: Please refer to Allen M, et al. Flow cytometry confirmed the expression of -hCG on U87MG cells. 8, Issue 354, pp. Important information: Please refer to Allen M, et al. The retrospective analysis looked at both the source tumour and original U-87MG cultures from the authors' laboratory in Uppsala . We find that the DNA profile of the widely used glioma cell line U87MG is different from that of the original cells and that it is likely to be a bona fide human glioblastoma cell line of unknown origin. It has been previously reported the COX-2 expression in several GBM cell lines, including U87MG and T98G, and the basal levels of this protein varied greatly among them [65, 66].To further confirm this evidence in our conditions, basal levels of COX-2 protein were evaluated in neurospheres generated from both U87MG and T98G cell lines. However, the U87MG cell line from ATCC may not be the original GBM cell line from the University of Uppsala established in 1968. GBM is an infiltrative tumor..using U87MG cell grafts we only obtained tumor bulck but no infiltrated cells..on a other hand this cell line seemed to be a good model to work on cancer stem . The human malignant meningioma IOMM-Lee cells were kindly provided by Dr. Anita Lal (University of California, San Francisco), the human malignant glioma U87MG cells, human osteosarcoma U-2OS and SAOS-2 cells, and estrogen-unresponsive breast carcinoma MDA-MB-231 cells were from the American Type Culture Collection (ATCC, Manassas, VA). In the present study, the morphologic and tumorigenicity of the cell strains were observed to change after serial passage.