No. The kits use starting sample volumes of up to 0.2 ml and combine the selective binding properties of a silica-based membrane with flexible elution volumes of For aorta, use Qiagens RNeasy Fibrous Tissue Mini Kit (Cat. You can search for protocols and protocol sheets by selecting an application, kit, or starting material. 74104) This kit will work on many tissues and cell types. For subsequent RNA Cleanup, use either the RNeasy MinElute Cleanup Kit, or follow the instructions for RNA Cleanup in You can search for protocols and protocol sheets by selecting an application, kit, or starting material. The kit uses QIAamp MinElute spin columns for purification of high-quality DNA with flexible elution volumes. The QIAwave RNA Mini Kit gives you highly reproducible yields of total RNA from animal or human cells, animal or human tissues, and yeast (see table Total RNA yields obtained with RNeasy Mini Spin Columns and figure " RT-PCR of RNA from as few as 100 cells ").The performance between our QIAwave RNA Mini Kit and RNeasy Mini Kit is identical because the Find out how to transfer protocols to your QIAcube This specialized protocol requires use of the RNeasy MinElute Cleanup Kit with the miRNeasy Micro or Mini Kit. 74104) This kit will work on many tissues and cell types. No. 74104) This kit will work on many tissues and cell types. RNA was isolated using the RNeasy Mini Kit (Qiagen). The samples were subsequently quantified and treated with gDNA Wipe-Out buffer (Qiagen). DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure " Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure " Laser microdissection PCR ").Purified DNA may also be used in short-tandem repeat DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure " Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure " Laser microdissection PCR ").Purified DNA may also be used in short-tandem repeat I used this protocol most extensively for gels that were ~400 microliters in volume and contained about 300,000 cells. My RNA samples have a 260/230 ratio of 10.21 and its 260/280 ratio is 2.58. Follow the standard tissue protocol in the RNeasy Lipid Tissue Kit Handbook. For a more refined search, select more than one parameter. The kits use starting sample volumes of up to 0.2 ml and combine the selective binding properties of a silica-based membrane with flexible elution volumes of DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure " Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure " Laser microdissection PCR ").Purified DNA may also be used in short-tandem repeat RNeasy mini kit: QIAGEN: 74106: Round bottom polystyrene tubes: Falcon: 352058: Materials and equipment. RNA amounts corresponding to less than one cell (as little as 1 pg) can be concentrated (see figure " Concentration of RNA ").A large amount of RNA (up to 45 g) can be purified and is suitable for Isolate RNA using a commercial kit such as Qiagen RNeasy Mini Kit or RNeasy Protect Bacteria Mini Kit. RNA extraction from cells and tissues with RNeasy Plus Micro and Mini Kits allows high, reproducible RNA yields and efficient genomic DNA elimination for sensitive applications (see figures Effective cell genomic DNA removal , Effective tissue genomic DNA removal , " High, reproducible RNA yields", and Array-ready RNA ). For aorta, use Qiagens RNeasy Fibrous Tissue Mini Kit (Cat. no. For each RT-PCR reaction, we used 50 to The column is then washed and pure, ready-to-use DNA is eluted. for Illumina sequencing according to the manufacturers protocol using the TruSeq stranded mRNA Sample Preparation Kit (Illumina, catalog no. 74104), according to manufacturers protocols. The kit uses QIAamp MinElute spin columns for purification of high-quality DNA with flexible elution volumes. The binding capacity of the spin-columns is up to 100 g RNA. Flow-through from the AllPrep DNA Mini spin column is digested by Proteinase K in the presence of ethanol. The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 10 7) and tissue samples (up to 50 mg).The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in The extracted RNA is then quantified using BioAnalyzer. The DNeasy 96 Blood & Tissue Kit provides high-throughput processing in a 96-well format using the QIAGEN 96-Well-Plate Centrifugation System. Total RNA with Agilent RIN values of close The QIAwave RNA Mini Kit gives you highly reproducible yields of total RNA from animal or human cells, animal or human tissues, and yeast (see table Total RNA yields obtained with RNeasy Mini Spin Columns and figure " RT-PCR of RNA from as few as 100 cells ").The performance between our QIAwave RNA Mini Kit and RNeasy Mini Kit is identical because the no. DNeasy PowerSoil Pro QIAcube Kit (240) QIAamp DNA Blood Mini QIAcube Kit (240) QIAamp DNA Mini QIAcube Kit (240) QIAamp PowerFecal Pro DNA QIAcube Kit (240) QIAamp Viral RNA Mini QIAcube Kit (240) RNeasy Mini QIAcube Kit (240) QIAcube Connect operates in conjunction with QIAcube Connect Accessories. The extracted RNA is then quantified using BioAnalyzer. Zhao N et al used RNeasy Mini Elute Cleanup Kit from QIAGEN to purify mRNA from in vitro transcribed T7-FB-GFP and T7-NHA-mCh-H2B using mMESSAGE mMACHINE kit from ThermoFisher Scientific for injection into zebrafish embryos . for Illumina sequencing according to the manufacturers protocol using the TruSeq stranded mRNA Sample Preparation Kit (Illumina, catalog no. The column is then washed and pure, ready-to-use DNA is eluted. With the RNeasy FFPE Kit, yields of RNA purified from fixed and embedded samples are greater than those achieved using other methods (see figure Greater yields of RNA ). My RNA samples have a 260/230 ratio of 10.21 and its 260/280 ratio is 2.58. For a more refined search, select more than one parameter. I used this protocol most extensively for gels that were ~400 microliters in volume and contained about 300,000 cells. Pick left primer, or use left primer below: Pick hybridization probe (internal oligo), or use oligo below: Pick right primer, or use right primer below (5' to 3' on opposite strand): The kit uses QIAamp MinElute spin columns for purification of high-quality DNA with flexible elution volumes. No more than 5 x 10 6 cells should be used. If using the miRNeasy 96 Kit, 2 RNeasy 96 plates are required for every 96 samples. A link to the relevant protocol sheet is provided in the detailed information about the protocol. RNA was extracted from the liver tissue using TRIzol (ThermoFisher Scientific, catalog no. The column is then washed and pure, ready-to-use DNA is eluted. Recovered RNA is intact down to 70 nucleotides in length and virtually free of genomic DNA contamination (see figure Recovery of all usable RNA ). When using the Midi or Maxi format, please follow the protocol for Isolation of DNA using the QIAamp DNA Blood Midi or Maxi Kit in the QIAamp Blood Midi and QIAamp Blood Maxi Handbook. No. This specialized protocol requires use of the RNeasy MinElute Cleanup Kit with the miRNeasy Micro or Mini Kit. The QIAamp Viral RNA Mini Kit (cat. The extracted RNA is then quantified using BioAnalyzer. If using the miRNeasy 96 Kit, 2 RNeasy 96 plates are required for every 96 samples. First-strand cDNA was prepared by using the QuanTect Kit (Qiagen). Usable RNA fragments purified with the kit from FFPE Zhao N et al used RNeasy Mini Elute Cleanup Kit from QIAGEN to purify mRNA from in vitro transcribed T7-FB-GFP and T7-NHA-mCh-H2B using mMESSAGE mMACHINE kit from ThermoFisher Scientific for injection into zebrafish embryos . QIAamp MinElute Virus Kits simplify viral DNA and RNA purification with fast spin-column and vacuum procedures. RNA extraction from cells and tissues with RNeasy Plus Micro and Mini Kits allows high, reproducible RNA yields and efficient genomic DNA elimination for sensitive applications (see figures Effective cell genomic DNA removal , Effective tissue genomic DNA removal , " High, reproducible RNA yields", and Array-ready RNA ). This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the RNeasy Mini spin column. No more than 5 x 10 6 cells should be used. The dedicated RNeasy Mini QIAcube Kit, including rotor adapters preloaded with RNeasy spin columns and elution tubes, delivers greater convenience and time savings (see figure "Significant time savings with dedicated QIAcube Kits").. Total RNA purified with the RNeasy Maxi Kit is of high quality and is suitable for many downstream applications (see figure "High-quality RNA For each RT-PCR reaction, we used 50 to For subsequent RNA Cleanup, use either the RNeasy MinElute Cleanup Kit, or follow the instructions for RNA Cleanup in Using the QuantiTect Reverse Transcription Kit, contaminating genomic DNA in RNA samples is effectively and rapidly removed with the unique gDNA Wipeout Buffer (see figure Effective genomic DNA removal for accurate real-time RT-PCR).Elimination of genomic DNA is crucial for accurate gene expression results, and design of RNA-specific primers or probes is not always 15596018) and RNeasy Mini Kit (Qiagen, catalog no. The QIAamp DNA Investigator Kit provides purification of genomic DNA from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. QIAamp MinElute Virus Kits simplify viral DNA and RNA purification with fast spin-column and vacuum procedures. The RNeasy Plant Mini Kit is ideal for isolation of total RNA from a wide variety of plant and fungal samples with sample sizes of 10100 mg tissue, or 1001 x 10 7 cells (see table "Selected samples processed with the RNeasy Plant Mini Kit"). If using the miRNeasy 96 Kit, 2 RNeasy 96 plates are required for every 96 samples. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. 74104), according to manufacturers protocols. No. A link to the relevant protocol sheet is provided in the detailed information about the protocol. Recommended: For isolating from cell culture and mouse liver, use Qiagens RNeasy Mini Kit (Cat. DNeasy Mini and 96 procedures. The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 10 7) and tissue samples (up to 50 mg).The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in DNeasy PowerSoil Pro QIAcube Kit (240) QIAamp DNA Blood Mini QIAcube Kit (240) QIAamp DNA Mini QIAcube Kit (240) QIAamp PowerFecal Pro DNA QIAcube Kit (240) QIAamp Viral RNA Mini QIAcube Kit (240) RNeasy Mini QIAcube Kit (240) QIAcube Connect operates in conjunction with QIAcube Connect Accessories. Please follow the Blood and Body Fluid Spin Protocol in the QIAamp DNA Mini and QIAamp DNA Blood Mini Kit Handbook. See figures. The binding capacity of the spin-columns is up to 100 g RNA. Usable RNA fragments purified with the kit from FFPE See figures. DNeasy Mini and 96 procedures. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. Using the QuantiTect Reverse Transcription Kit, contaminating genomic DNA in RNA samples is effectively and rapidly removed with the unique gDNA Wipeout Buffer (see figure Effective genomic DNA removal for accurate real-time RT-PCR).Elimination of genomic DNA is crucial for accurate gene expression results, and design of RNA-specific primers or probes is not always The dedicated RNeasy Mini QIAcube Kit, including rotor adapters preloaded with RNeasy spin columns and elution tubes, delivers greater convenience and time savings (see figure "Significant time savings with dedicated QIAcube Kits").. Total RNA purified with the RNeasy Maxi Kit is of high quality and is suitable for many downstream applications (see figure "High-quality RNA The RNeasy MinElute Cleanup Kit provides high-quality total RNA, free from impurities or enzymatic inhibitors, with A 260 /A 280 ratios of 1.92.1 (see figure " High-quality RNA "). Flow-through from the AllPrep DNA Mini spin column is digested by Proteinase K in the presence of ethanol. The QIAamp DNA Investigator Kit provides purification of genomic DNA from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. Enrichment of small RNAs in a separate fraction may be advantageous for certain applications where mRNA and rRNA could lead to increased background. Finally, the cell lysate was combined with 350 L of RLT plus buffer, and processed according to the RNeasy Plus Mini Kits protocol. RNA was isolated using the RNeasy Mini Kit (Qiagen). Recovered RNA is intact down to 70 nucleotides in length and virtually free of genomic DNA contamination (see figure Recovery of all usable RNA ). RNA was extracted from the liver tissue using TRIzol (ThermoFisher Scientific, catalog no. no. RNeasy mini kit: QIAGEN: 74106: Round bottom polystyrene tubes: Falcon: 352058: Materials and equipment. Enrichment of small RNAs in a separate fraction may be advantageous for certain applications where mRNA and rRNA could lead to increased background. I am extracting total RNA from bacteria by using Trizol and purified it by using the RNeasy Mini Kit from QIAGEN. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report. The QIAamp Viral RNA Mini Kit (cat. Follow the standard tissue protocol in the RNeasy Lipid Tissue Kit Handbook. I am extracting total RNA from bacteria by using Trizol and purified it by using the RNeasy Mini Kit from QIAGEN. RNeasy variations include RNeasy Plus Mini Kit [45, 103] and RNeasy Micro kit , among others. Finally, the cell lysate was combined with 350 L of RLT plus buffer, and processed according to the RNeasy Plus Mini Kits protocol. For a more refined search, select more than one parameter. Yes. The RNeasy Fibrous Tissue Mini Kit includes proteinase K for removing abundant protein in fiber-rich tissue samples, and RNeasy spin columns for purifying up to 100 g of high-quality RNA. The RNeasy Fibrous Tissue Mini Kit includes proteinase K for removing abundant protein in fiber-rich tissue samples, and RNeasy spin columns for purifying up to 100 g of high-quality RNA. Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report. The QIAamp DNA Investigator Kit provides purification of genomic DNA from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. Using the QuantiTect Reverse Transcription Kit, contaminating genomic DNA in RNA samples is effectively and rapidly removed with the unique gDNA Wipeout Buffer (see figure Effective genomic DNA removal for accurate real-time RT-PCR).Elimination of genomic DNA is crucial for accurate gene expression results, and design of RNA-specific primers or probes is not always You can search for protocols and protocol sheets by selecting an application, kit, or starting material. The QIAwave RNA Mini Kit gives you highly reproducible yields of total RNA from animal or human cells, animal or human tissues, and yeast (see table Total RNA yields obtained with RNeasy Mini Spin Columns and figure " RT-PCR of RNA from as few as 100 cells ").The performance between our QIAwave RNA Mini Kit and RNeasy Mini Kit is identical because the With the RNeasy FFPE Kit, yields of RNA purified from fixed and embedded samples are greater than those achieved using other methods (see figure Greater yields of RNA ). For subsequent RNA Cleanup, use either the RNeasy MinElute Cleanup Kit, or follow the instructions for RNA Cleanup in Pick left primer, or use left primer below: Pick hybridization probe (internal oligo), or use oligo below: Pick right primer, or use right primer below (5' to 3' on opposite strand): Each step of the protocol execution is recorded to accelerate reporting by generating a comprehensive run report. Yes. Total RNA with Agilent RIN values of close The RNeasy Fibrous Tissue Mini Kit includes proteinase K for removing abundant protein in fiber-rich tissue samples, and RNeasy spin columns for purifying up to 100 g of high-quality RNA. The RNeasy Plant Mini Kit is ideal for isolation of total RNA from a wide variety of plant and fungal samples with sample sizes of 10100 mg tissue, or 1001 x 10 7 cells (see table "Selected samples processed with the RNeasy Plant Mini Kit"). The QIAamp Viral RNA Mini Kit (cat. The RNeasy Mini Kit, RNeasy Protect Mini Kit (including Tissue ReagentRNAprotect), and RNeasy Plant Mini Kit should be stored dry at room temperature (15 25C) and are stable for at least 9 months under these conditions, if not otherwise stated on the label. The Quick-RNA Miniprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 10 7) and tissue samples (up to 50 mg).The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in The RNeasy Plant Mini Kit is ideal for isolation of total RNA from a wide variety of plant and fungal samples with sample sizes of 10100 mg tissue, or 1001 x 10 7 cells (see table "Selected samples processed with the RNeasy Plant Mini Kit"). Recommended: For isolating from cell culture and mouse liver, use Qiagens RNeasy Mini Kit (Cat. The DNeasy 96 Blood & Tissue Kit provides high-throughput processing in a 96-well format using the QIAGEN 96-Well-Plate Centrifugation System. Zhao N et al used RNeasy Mini Elute Cleanup Kit from QIAGEN to purify mRNA from in vitro transcribed T7-FB-GFP and T7-NHA-mCh-H2B using mMESSAGE mMACHINE kit from ThermoFisher Scientific for injection into zebrafish embryos . The dedicated RNeasy Mini QIAcube Kit, including rotor adapters preloaded with RNeasy spin columns and elution tubes, delivers greater convenience and time savings (see figure "Significant time savings with dedicated QIAcube Kits").. Total RNA purified with the RNeasy Maxi Kit is of high quality and is suitable for many downstream applications (see figure "High-quality RNA A protocol for in-solution DNase digestion using the RNase-Free DNase Set can be found in Appendix C of the RNeasy MinElute Cleanup Handbook. RNA amounts corresponding to less than one cell (as little as 1 pg) can be concentrated (see figure " Concentration of RNA ").A large amount of RNA (up to 45 g) can be purified and is suitable for My RNA samples have a 260/230 ratio of 10.21 and its 260/280 ratio is 2.58. I used this protocol most extensively for gels that were ~400 microliters in volume and contained about 300,000 cells. QIAamp MinElute Virus Kits simplify viral DNA and RNA purification with fast spin-column and vacuum procedures. RNA was extracted from the liver tissue using TRIzol (ThermoFisher Scientific, catalog no. Isolate RNA using a commercial kit such as Qiagen RNeasy Mini Kit or RNeasy Protect Bacteria Mini Kit. Recovered RNA is intact down to 70 nucleotides in length and virtually free of genomic DNA contamination (see figure Recovery of all usable RNA ). The samples were subsequently quantified and treated with gDNA Wipe-Out buffer (Qiagen). RNeasy variations include RNeasy Plus Mini Kit [45, 103] and RNeasy Micro kit , among others. A protocol for in-solution DNase digestion using the RNase-Free DNase Set can be found in Appendix C of the RNeasy MinElute Cleanup Handbook. Total RNA with Agilent RIN values of close 15596018) and RNeasy Mini Kit (Qiagen, catalog no. The binding capacity of the spin-columns is up to 100 g RNA. Please follow the Blood and Body Fluid Spin Protocol in the QIAamp DNA Mini and QIAamp DNA Blood Mini Kit Handbook. RNA extraction from cells and tissues with RNeasy Plus Micro and Mini Kits allows high, reproducible RNA yields and efficient genomic DNA elimination for sensitive applications (see figures Effective cell genomic DNA removal , Effective tissue genomic DNA removal , " High, reproducible RNA yields", and Array-ready RNA ). Please follow the Blood and Body Fluid Spin Protocol in the QIAamp DNA Mini and QIAamp DNA Blood Mini Kit Handbook. When using the Midi or Maxi format, please follow the protocol for Isolation of DNA using the QIAamp DNA Blood Midi or Maxi Kit in the QIAamp Blood Midi and QIAamp Blood Maxi Handbook. The RNeasy Mini Kit, RNeasy Protect Mini Kit (including Tissue ReagentRNAprotect), and RNeasy Plant Mini Kit should be stored dry at room temperature (15 25C) and are stable for at least 9 months under these conditions, if not otherwise stated on the label. Usable RNA fragments purified with the kit from FFPE A protocol for in-solution DNase digestion using the RNase-Free DNase Set can be found in Appendix C of the RNeasy MinElute Cleanup Handbook. No. The samples were subsequently quantified and treated with gDNA Wipe-Out buffer (Qiagen). Yes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the RNeasy Mini spin column. RNeasy variations include RNeasy Plus Mini Kit [45, 103] and RNeasy Micro kit , among others. Recommended: For isolating from cell culture and mouse liver, use Qiagens RNeasy Mini Kit (Cat. for Illumina sequencing according to the manufacturers protocol using the TruSeq stranded mRNA Sample Preparation Kit (Illumina, catalog no. 15596018) and RNeasy Mini Kit (Qiagen, catalog no. Isolate RNA using a commercial kit such as Qiagen RNeasy Mini Kit or RNeasy Protect Bacteria Mini Kit. DNeasy Mini and 96 procedures. RNA amounts corresponding to less than one cell (as little as 1 pg) can be concentrated (see figure " Concentration of RNA ").A large amount of RNA (up to 45 g) can be purified and is suitable for This specialized protocol requires use of the RNeasy MinElute Cleanup Kit with the miRNeasy Micro or Mini Kit. With the RNeasy FFPE Kit, yields of RNA purified from fixed and embedded samples are greater than those achieved using other methods (see figure Greater yields of RNA ). RNeasy mini kit: QIAGEN: 74106: Round bottom polystyrene tubes: Falcon: 352058: Materials and equipment. The RNeasy MinElute Cleanup Kit provides high-quality total RNA, free from impurities or enzymatic inhibitors, with A 260 /A 280 ratios of 1.92.1 (see figure " High-quality RNA "). No more than 5 x 10 6 cells should be used. For aorta, use Qiagens RNeasy Fibrous Tissue Mini Kit (Cat. DNeasy PowerSoil Pro QIAcube Kit (240) QIAamp DNA Blood Mini QIAcube Kit (240) QIAamp DNA Mini QIAcube Kit (240) QIAamp PowerFecal Pro DNA QIAcube Kit (240) QIAamp Viral RNA Mini QIAcube Kit (240) RNeasy Mini QIAcube Kit (240) QIAcube Connect operates in conjunction with QIAcube Connect Accessories. The RNeasy Mini Kit, RNeasy Protect Mini Kit (including Tissue ReagentRNAprotect), and RNeasy Plant Mini Kit should be stored dry at room temperature (15 25C) and are stable for at least 9 months under these conditions, if not otherwise stated on the label. Finally, the cell lysate was combined with 350 L of RLT plus buffer, and processed according to the RNeasy Plus Mini Kits protocol. Enrichment of small RNAs in a separate fraction may be advantageous for certain applications where mRNA and rRNA could lead to increased background. First-strand cDNA was prepared by using the QuanTect Kit (Qiagen). Find out how to transfer protocols to your QIAcube Flow-through from the AllPrep DNA Mini spin column is digested by Proteinase K in the presence of ethanol. Follow the standard tissue protocol in the RNeasy Lipid Tissue Kit Handbook. See figures. When using the Midi or Maxi format, please follow the protocol for Isolation of DNA using the QIAamp DNA Blood Midi or Maxi Kit in the QIAamp Blood Midi and QIAamp Blood Maxi Handbook. Find out how to transfer protocols to your QIAcube RNA was isolated using the RNeasy Mini Kit (Qiagen). I am extracting total RNA from bacteria by using Trizol and purified it by using the RNeasy Mini Kit from QIAGEN. The DNeasy 96 Blood & Tissue Kit provides high-throughput processing in a 96-well format using the QIAGEN 96-Well-Plate Centrifugation System. The kits use starting sample volumes of up to 0.2 ml and combine the selective binding properties of a silica-based membrane with flexible elution volumes of This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the RNeasy Mini spin column. A link to the relevant protocol sheet is provided in the detailed information about the protocol. Pick left primer, or use left primer below: Pick hybridization probe (internal oligo), or use oligo below: Pick right primer, or use right primer below (5' to 3' on opposite strand): 74104), according to manufacturers protocols. For each RT-PCR reaction, we used 50 to First-strand cDNA was prepared by using the QuanTect Kit (Qiagen). No. The RNeasy MinElute Cleanup Kit provides high-quality total RNA, free from impurities or enzymatic inhibitors, with A 260 /A 280 ratios of 1.92.1 (see figure " High-quality RNA ").